This three-laser (405nm, 488nm and 640nm) flow cytometer has multiple bandpass filters for analysis of up to 13 parameters. The instrument requires easy daily maintenance with automated start-up, QC and shutdown procedures. Data is analyzed using IDEAS® software, combining image analysis and statistical stringency, which is loaded on separate workstation. cell signaling and intensity, internalization, cell cycle, morphology, cell-cell interaction, co-localization, autophagy, etc). It has numerous possible applications for qualitative and quantitative measurements (i.e. The fluidics system provides up to 95 percent sample utilization (for when you need every cell) or the ability to return the remaining sample for additional applications. The instrument can run up to 5,000 cells/sec (ideal for rare cell analysis) and uses less volume and less waste. It is ideal for many applications including monitoring cell signaling and intensity, internalization, cell cycle, morphology, cell-cell interaction, co-localization and autophagy. The instrument has multiple magnifications with 20X/40X/60X objectives (motorized and autofocused) along with an extended depth of field function using optics and algorithms to project structures within a cell into one focal plane (i.e., subcellular features, fluorescent in situ hybridization spots, autophagy, nuclear translocation, etc). It has excitation lasers at 405nm, 488nm and 642nm and allows for 12 high-resolution images of every event in the flow cell, including bright field, dark field and 10 fluorescent channels.
This flow cytometer combines the phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. Our facility has the following instruments:Īmnis® ImageStream® Mark II Imaging Flow Cytometer
Our suite of multi-parameter flow cytometers are well equipped for fluorescence-activated cell sorting (FACS) and emerging flow cytometry assays. The facility provides the latest technology and professional technical assistance for flow cytometric analysis and sorting. The Institute for Immunology now offers latest in flow cytometry services at our Flow Core Facility. Please visit the new Flow Core website at. Institute for Immunology > Flow Cytometry Facility Flow Cytometry Services
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